abstract：:Surfactant protein (SP)-D, a 43-kD multifunctional collagen-like lectin, is synthesized and secreted by the airway epithelium. SP-D knockout (SP-D [-/-]) mice exhibit an increase in the number and size of airway macrophages, peribronchiolar inflammation, increases in metalloproteinase activity, and development of emph...

journal_title：American journal of respiratory cell and molecular biology

authors： Atochina EN,Beers MF,Hawgood S,Poulain F,Davis C,Fusaro T,Gow AJ

更新日期：2004-03-01 00:00:00

abstract：:Gene therapy requires the presence of a robust and yet small promoter to drive high-level expression of desired proteins. In comparative analysis, we investigated the promoter strength of the CTP:phosphocholine cytidylyltransferase promoter (CCT alpha) with other commonly used promoters, which were all cloned into a s...

journal_title：American journal of respiratory cell and molecular biology

authors： Zhou J,You Y,Zabner J,Ryan AJ,Mallampalli RK

更新日期：2004-01-01 00:00:00

abstract：:We investigated the expression and function of matrix metalloproteinase-12 (MMP-12) in a model of allergic airway inflammation. Mice were sensitized mucosally by exposure to aerosolized ovalbumin (OVA) daily over a period of 10 d in the context of adenovirus-mediated granulocyte macrophage colony-stimulating factor (G...

journal_title：American journal of respiratory cell and molecular biology

authors： Pouladi MA,Robbins CS,Swirski FK,Cundall M,McKenzie AN,Jordana M,Shapiro SD,Stämpfli MR

更新日期：2004-01-01 00:00:00

abstract：:The purpose of this study was to characterize basolateral anion channels in Calu-3 and normal human bronchial epithelial cells, and their role in anion secretion. Patch clamp studies identified an outwardly rectifying Cl- channel (ORCC), which could be activated by the adenosine receptor agonist 5'-(N-ethylcarboxamido...

journal_title：American journal of respiratory cell and molecular biology

authors： Szkotak AJ,Man SF,Duszyk M

更新日期：2003-12-01 00:00:00

abstract：:Tumor necrosis factor-alpha (TNF-alpha) is thought to be important in the development of pulmonary fibrosis. However, surfactant protein-C/TNF-alpha transgenic mice do not spontaneously develop pulmonary fibrosis but instead develop alveolar enlargement and loss of elastic recoil. We hypothesized that overexpression o...

journal_title：American journal of respiratory cell and molecular biology

authors： Fujita M,Shannon JM,Morikawa O,Gauldie J,Hara N,Mason RJ

更新日期：2003-12-01 00:00:00

abstract：:The mammalian alveolar epithelium is composed of alveolar type I (AT1) and alveolar type II (AT2) cells that together coordinate tissue function. We used a heterocellular culture model of AT1 and AT2 cells to determine pathways for intercellular signaling between these two phenotypes. Gap junction protein (connexin) p...

journal_title：American journal of respiratory cell and molecular biology

authors： Isakson BE,Seedorf GJ,Lubman RL,Evans WH,Boitano S

更新日期：2003-11-01 00:00:00

abstract：:We examined the mechanisms underlying tachyphylaxis to beta-adrenergic receptor agonists (beta-agonists) in tracheal smooth muscle. Simultaneous measurements of isometric tension and intracellular Ca2+ concentration ([Ca2+]i) using fura-2-loaded guinea pig tracheas showed that the inhibitory effects of isoproterenol (...

journal_title：American journal of respiratory cell and molecular biology

authors： Kume H,Ishikawa T,Oguma T,Ito S,Shimokata K,Kotlikoff MI

更新日期：2003-09-01 00:00:00

abstract：:Endothelin-1 (ET-1) exerts many biological effects in airways, including bronchoconstriction, airway mucus secretion, cell proliferation, and inflammation. We investigated the effect of ET-1 on Na absorption and Cl secretion in human bronchial epithelial cells. Addition of 10(-7) M ET-1 had no effect on the inhibition...

journal_title：American journal of respiratory cell and molecular biology

authors： Blouquit S,Sari A,Lombet A,D'herbomez M,Naline E,Matran R,Chinet T

更新日期：2003-08-01 00:00:00

abstract：:Hyperplasia and cell migration of smooth muscle are features of both airway and pulmonary vascular diseases. The precise cellular and molecular mechanisms that regulate smooth muscle migration in the lungs remain unknown. In this study, we examined the effect of cAMP-mobilizing agents and steroids on smooth muscle cel...

journal_title：American journal of respiratory cell and molecular biology

authors： Goncharova EA,Billington CK,Irani C,Vorotnikov AV,Tkachuk VA,Penn RB,Krymskaya VP,Panettieri RA Jr

更新日期：2003-07-01 00:00:00

abstract：:Enzymes which exhibit core 2 beta1,6 N-acetylglucosaminyltransferase (C2GnT) activity play important roles in physiologic processes including the inflammatory response and immune system function, and C2GnT activity is regulated during processes, such as T cell activation and cellular differentiation. In this study, we...

journal_title：American journal of respiratory cell and molecular biology

authors： Beum PV,Bastola DR,Cheng PW

更新日期：2003-07-01 00:00:00

abstract：:Many of the fibrogenic effects of transforming growth factor-beta (TGF-beta) might be mediated by connective tissue growth factor (CTGF). The present study investigates the role of mitogen-activated protein (MAP) kinase in the expression of CTGF mRNA in the human lung fibroblast line, HFL-1. TGF-beta1 enhanced CTGF mR...

journal_title：American journal of respiratory cell and molecular biology

authors： Utsugi M,Dobashi K,Ishizuka T,Masubuchi K,Shimizu Y,Nakazawa T,Mori M

更新日期：2003-06-01 00:00:00

abstract：:Eotaxins-1, -2, and -3 mediate the recruitment of blood-borne eosinophils to allergically inflamed tissues by binding CC chemokine receptor (CCR) 3. Mast cells (MCs) are resident tissue cells that also express CCR3. In the present study, we demonstrate that human (h) MCs in nasal polyps and cultured cord blood-derived...

journal_title：American journal of respiratory cell and molecular biology

authors： Price KS,Friend DS,Mellor EA,De Jesus N,Watts GF,Boyce JA

更新日期：2003-04-01 00:00:00

abstract：:Pulmonary lymphangioleiomyomatosis (LAM) is characterized by abnormal smooth muscle-like cell proliferation leading to tissue destruction and cyst formation. We demonstrate that serum response factor (SRF), a critical smooth muscle transcription factor, is overexpressed in LAM cells. To determine whether abnormal SRF ...

journal_title：American journal of respiratory cell and molecular biology

authors： Zhe X,Yang Y,Jakkaraju S,Schuger L

更新日期：2003-04-01 00:00:00

abstract：:Proteinase-activated receptor (PAR)-2 is cleaved within its aminoterminal extracellular domain by serine proteinases such as trypsin, unmasking a new aminoterminus starting with the sequence SLIGKV, which binds intramolecularly and activates the receptor. PAR-2 has been reported to be involved in inflammation within t...

journal_title：American journal of respiratory cell and molecular biology

authors： Dulon S,Candé C,Bunnett NW,Hollenberg MD,Chignard M,Pidard D

更新日期：2003-03-01 00:00:00

abstract：:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL/APO-2L) is a member of the tumor necrosis factor family that induces apoptosis in a variety of transformed cell lines and in normal human hepatocytes and brain cells. Soluble TRAIL at high concentrations was found to induce apoptotic death in normal human ...

journal_title：American journal of respiratory cell and molecular biology

authors： Yurovsky VV

更新日期：2003-02-01 00:00:00

abstract：:The glucocorticoids (GC) and retinoids (RA) modulate branching morphogenesis and cytodifferentiation in the developing lung. We investigated downstream target genes that link glucocorticoid stimulation to the achievement of a mature lung in glucocorticoid receptor (GR) knockout mice. All GR(null) mice and approximatel...

journal_title：American journal of respiratory cell and molecular biology

authors： Kaplan F,Comber J,Sladek R,Hudson TJ,Muglia LJ,Macrae T,Gagnon S,Asada M,Brewer JA,Sweezey NB

更新日期：2003-01-01 00:00:00

abstract：:Pulmonary fibrosis (PF) is caused by a number of inhaled agents, as well as by some drugs and toxic particles. The elaboration of certain peptide growth factors is thought to be key to the development of this disease process. In addition, genetic susceptibility plays a role in the development of PF. For instance, we h...

journal_title：American journal of respiratory cell and molecular biology

authors： Warshamana GS,Pociask DA,Sime P,Schwartz DA,Brody AR

更新日期：2002-12-01 00:00:00

abstract：:Important characteristics of chronic obstructive pulmonary disease (COPD) include airway and vascular remodeling, the molecular mechanisms of which are poorly understood. We assessed the role of fibroblast growth factors (FGF) in pulmonary vascular remodeling by examining the expression pattern of FGF-1, FGF-2, and th...

journal_title：American journal of respiratory cell and molecular biology

authors： Kranenburg AR,De Boer WI,Van Krieken JH,Mooi WJ,Walters JE,Saxena PR,Sterk PJ,Sharma HS

更新日期：2002-11-01 00:00:00

abstract：:The gaseous molecule carbon monoxide (CO) is elevated in the breath of individuals with asthma. The physiologic function of CO in asthma is poorly understood. Here we demonstrate that CO (250 ppm) markedly inhibits human airway smooth muscle cell (HASMC) proliferation, arresting cells at the G0/G1 phase. This CO-induc...

journal_title：American journal of respiratory cell and molecular biology

authors： Song R,Mahidhara RS,Liu F,Ning W,Otterbein LE,Choi AM

更新日期：2002-11-01 00:00:00

abstract：:Inflammatory lung diseases are associated with increased production of matrix metalloproteinase-9 (MMP-9) from infiltrating granulocytes or from the respiratory epithelium, and inappropriate expression and activation of MMP-9 may be associated with tissue injury and airway remodeling. Inflammatory conditions also resu...

journal_title：American journal of respiratory cell and molecular biology

authors： Okamoto T,Valacchi G,Gohil K,Akaike T,van der Vliet A

更新日期：2002-10-01 00:00:00

abstract：:Zn may have an important protective role in the respiratory epithelium and Zn deficiency may enhance airway inflammation and epithelial damage. The effects of mild nutritional Zn deficiency on airway hyperresponsiveness (AHR) and airway inflammation in mice sensitized and challenged with ovalbumin (OVA) to induce an a...

journal_title：American journal of respiratory cell and molecular biology

authors： Truong-Tran AQ,Ruffin RE,Foster PS,Koskinen AM,Coyle P,Philcox JC,Rofe AM,Zalewski PD

更新日期：2002-09-01 00:00:00

abstract：:The cells/proteases responsible for the development of smoke-induced emphysema is an area of intense investigation. Mice with knockout of macrophage metalloelastase genes (MME(-/-)) do not develop emphysema after smoke exposure, but we also observed that neutrophils (PMN) in lavage appeared to be a requirement for acu...

journal_title：American journal of respiratory cell and molecular biology

authors： Churg A,Zay K,Shay S,Xie C,Shapiro SD,Hendricks R,Wright JL

更新日期：2002-09-01 00:00:00

abstract：:Mutations in the p53 gene have been implicated to play an important role in the development of various human cancers. To evaluate the importance of p53 in lung cancer, a transgenic mouse model was established by utilizing the Clara cell secretory protein (CCSP) promoter to target the expression of a dominant-negative ...

journal_title：American journal of respiratory cell and molecular biology

authors： Tchou-Wong KM,Jiang Y,Yee H,LaRosa J,Lee TC,Pellicer A,Jagirdar J,Gordon T,Goldberg JD,Rom WN

更新日期：2002-08-01 00:00:00

abstract：:Organisms on our planet have evolved in an oxidizing environment that is intrinsically inimical to life, and cells have been forced to devise means of protecting themselves. One of the defenses used most widely in nature is the enzyme heme oxygenase-1 (HO-1). This enzyme performs the seemingly lackluster function of c...

journal_title：American journal of respiratory cell and molecular biology

authors： Morse D,Choi AM

更新日期：2002-07-01 00:00:00

abstract：:Airway neutrophilia is a prominent feature of chronic obstructive pulmonary disease. As cigarette smoke (CS) and epidermal growth factor (EGF) both cause release of interleukin-8 (IL-8) from epithelial cells in vitro, we investigated whether autocrine ligands for the EGF receptor (EGFR) are involved in this proinflamm...

journal_title：American journal of respiratory cell and molecular biology

authors： Richter A,O'Donnell RA,Powell RM,Sanders MW,Holgate ST,Djukanović R,Davies DE

更新日期：2002-07-01 00:00:00

abstract：:CTP:phosphocholine cytidylyltransferase (CT) is the rate-limiting enzyme in the biosynthesis by type II pneumocytes of phosphatidylcholine (PC), the predominant phospholipid in lung surfactant. Augmentation of endogenous CT activity might therefore result in enhanced surfactant PC production. To test this hypothesis, ...

journal_title：American journal of respiratory cell and molecular biology

authors： Li J,Marsh JJ,Spragg RG

更新日期：2002-06-01 00:00:00

abstract：:To better understand asthma pathogenesis, we characterized airway responsiveness, lung inflammation, and mediator production of alveolar macrophages (AM) after allergen sensitization and challenge in two strains of rats showing different susceptibilities in developing airway allergic reactions. Airway responsiveness t...

journal_title：American journal of respiratory cell and molecular biology

authors： Careau E,Sirois J,Bissonnette EY

更新日期：2002-05-01 00:00:00

abstract：:Modification of tyrosine residues and formation of 3-nitrotyrosine is one of the most commonly identified effects of reactive nitrogen species on proteins. In this study we evaluated the presence and localization of tyrosine nitration in various ventilatory and limb muscles. We also assessed the contribution of the ne...

journal_title：American journal of respiratory cell and molecular biology

authors： Barreiro E,Comtois AS,Gea J,Laubach VE,Hussain SN

更新日期：2002-04-01 00:00:00

abstract：:Human alveolar macrophages (HAM) express FcalphaR receptors for immunoglobulin (Ig)A which could link humoral and cellular branches of lung immunity. Here, we investigate the effects of polymeric (p-IgA) and secretory (S-IgA) IgA interaction with Fc(alpha)R on lipopolysaccharide (LPS)- and phorbol myristate acetate (P...

journal_title：American journal of respiratory cell and molecular biology

authors： Ouadrhiri Y,Pilette C,Monteiro RC,Vaerman JP,Sibille Y

更新日期：2002-03-01 00:00:00

abstract：:Primary pulmonary hypertension is characterized by increased pulmonary vascular resistance and smooth muscle proliferation. Stable analogs are increasingly being used to treat this disease, although no data exists comparing their effects on proliferation. We therefore investigated the antiproliferative activity of sev...

journal_title：American journal of respiratory cell and molecular biology

authors： Clapp LH,Finney P,Turcato S,Tran S,Rubin LJ,Tinker A

更新日期：2002-02-01 00:00:00

abstract：:The effects of hypoxia on the regulation of inducible nitric oxide synthase (NOS) 2 expression were examined in cultured rat pulmonary microvascular endothelial cells (EC). EC did not express NOS 2 mRNA or protein when exposed to normoxia or hypoxia unless they were pretreated with interleukin (IL)-1beta and/or tumor ...

journal_title：American journal of respiratory cell and molecular biology

authors： Zulueta JJ,Sawhney R,Kayyali U,Fogel M,Donaldson C,Huang H,Lanzillo JJ,Hassoun PM

更新日期：2002-01-01 00:00:00

abstract：:To examine the effects of acid exposure with moderate acidity (pH 3.0-5.0) on bactericidal activity of airway surface liquid (ASL), ASL was collected by washing the surface of primary cultures of human tracheal epithelial cells 24 h after treatment with phosphate-buffered saline (PBS) adjusted to a pH of 3.0, 4.0, or ...

journal_title：American journal of respiratory cell and molecular biology

authors： Nakayama K,Jia YX,Hirai H,Shinkawa M,Yamaya M,Sekizawa K,Sasaki H

更新日期：2002-01-01 00:00:00

abstract：:Remodeling of the airways, as occurs in asthmatic patients, is associated with the continual presence of inflammatory mediators and Th2 cytokines, especially interleukin (IL)-13, during cycles of epithelial injury and repair. In this study, we examined the effect of IL-13 on well-differentiated normal human bronchial ...

journal_title：American journal of respiratory cell and molecular biology

authors： Booth BW,Adler KB,Bonner JC,Tournier F,Martin LD

更新日期：2001-12-01 00:00:00

abstract：:Previous studies have demonstrated that high tidal volumes can cause interstitial and alveolar edema, with degradation of pulmonary epithelial barrier integrity. Separate studies have shown that F-actin disruption and decreased intracellular ATP (ATP(i)) levels in the nonpulmonary epithelium can increase tight junctio...

journal_title：American journal of respiratory cell and molecular biology

authors： Cavanaugh KJ Jr,Oswari J,Margulies SS

更新日期：2001-11-01 00:00:00

abstract：:Opiate-like peptides can regulate many cellular functions. We now map [D-Ala(2)]deltorphin I (DADTI)-like immunoreactivity (DADTI-LI) in developing mouse lung and analyze potential functional roles. Most DADTI-LI-positive cells were alveolar cells negative for prosurfactant protein (proSP)-C immunoreactivity. Peak num...

journal_title：American journal of respiratory cell and molecular biology

authors： Sunday ME,Haley KJ,Emanuel RL,Torday JS,Asokananthan N,Sikorski KA,Tooyama I,Kimura H,Renda T,Erspamer V

更新日期：2001-10-01 00:00:00

abstract：:The objective of this study was to define phenotypic changes of antigen-presenting cells (APCs) and T cells in a murine model of antigen-induced airways inflammation that involves intraperitoneal sensitization with ovalbumin (OVA)/adjuvant followed by antigen aerosolization. We investigated the APC and T-cell compartm...

journal_title：American journal of respiratory cell and molecular biology

authors： Gajewska BU,Swirski FK,Alvarez D,Ritz SA,Goncharova S,Cundall M,Snider DP,Coyle AJ,Gutierrez-Ramos JC,Stämpfli MR,Jordana M

更新日期：2001-09-01 00:00:00

abstract：:beta-glucans represent major structural components of fungal cell walls. We recently reported that Pneumocystis carinii beta-glucans stimulate alveolar macrophages to release proinflammatory cytokines. Macrophage activation by beta-glucan is augmented by serum, implying the presence of circulating factors that interac...

journal_title：American journal of respiratory cell and molecular biology

authors： Vassallo R,Kottom TJ,Standing JE,Limper AH

更新日期：2001-08-01 00:00:00

abstract：:Chloride (Cl(-)) movement across fetal lung epithelia is thought to be mediated by the sodium-potassium-2-Cl(-) cotransporter NKCC1. We studied the role of NKCC1 in Cl(-) and liquid secretion in late-gestation NKCC-null (-/-) and littermate control fetal mouse lung. NKCC -/- mice had decreased lung water compared with...

journal_title：American journal of respiratory cell and molecular biology

authors： Gillie DJ,Pace AJ,Coakley RJ,Koller BH,Barker PM

更新日期：2001-07-01 00:00:00

abstract：:Geldanamycin is a benzoquinone ansamycin with multiple pharmacologic properties. Recent data demonstrated that geldanamycin conferred protection in an animal model of inflammation-associated acute lung injury. In the current study, we investigated the effects of geldanamycin on interleukin (IL)-8 gene expression and n...

journal_title：American journal of respiratory cell and molecular biology

authors： Malhotra V,Shanley TP,Pittet JF,Welch WJ,Wong HR

更新日期：2001-07-01 00:00:00

abstract：:It is generally important to elucidate airway epithelial cell lineages and to identify multipotent progenitors as targets for gene therapy. Stem (S) cells are typically present in specialized compartments spatially proximal to their differentiated progeny, but an equivalent paradigm has not been demonstrated in the ai...

journal_title：American journal of respiratory cell and molecular biology

authors： Borthwick DW,Shahbazian M,Krantz QT,Dorin JR,Randell SH

更新日期：2001-06-01 00:00:00